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Table 4 Reproducibility of QSS on mixtures of plasmid DNA

From: Quantitative Single-letter Sequencing: a method for simultaneously monitoring numerous known allelic variants in single DNA samples

  Percentage of each VIT Total (%)
Replicates of Mix N°9 VIT1 VIT2 VIT3 VIT4 VIT5 VIT6  
1 16.92 16.78 16.55 16.72 16.59 16.43 100
2 17.45 16.99 17.14 16.12 16.19 16.12 100
3 17.17 16.58 16.81 16.74 16.79 15.92 100
4 17.06 16.47 16.32 16.68 17.33 16.14 100
5 17.19 16.36 17.03 16.60 17.21 15.62 100
Mean 17,16 16,63 16,77 16,57 16,82 16,05 100
SD 0.195 0.251 0.336 0.261 0.465 0.301  
  1. Reproducibility of QSS as evaluated by the standard deviation (SD) on 5 replications of the analysis of Mix n°9. Proportional correction of the frequency of all markers was applied to give a total of 100% in each mix.