Multiple sequence alignment of wheat nsLTPs. Amino acid sequences were deduced from genes or ESTs indexed in the NCBI database. Amino acid sequences were aligned using HMMERalign to maximize the eight-cysteine motif alignment, and manually refined. For each nsLTP subfamily, one sequence is presented and the number of putative members identified is indicated between parentheses. The conserved cysteine residues are black boxed and additional cysteine residues grey boxed. Accession numbers are given in Additional file 2 and amino acid sequence of mature nsLTPs in Additional file 3.