Developmentally regulated expression, alternative splicing and distinct sub-groupings in members of the Schistosoma mansoni venom allergen-like (SmVAL) gene family

Table 2 Molecular Details/Characteristics of SmVAL14-28.

SmVAL^a	PCR confirmation^b	S. mansoni v4 ID^c	Protein size (AA)^d	Gene length (bp)^e	Signal peptide^f	Conserved intron phase^g	Gp1/2^h
14	Yes	smp_078490	219	1514	Yes	0/2/0/2	1
15	Yes	smp_070250	270	4387	No**	0/2/0/2	1
16	Yes	smp_124070	169	25196	No	1/1	2
17	No	smp_163400	168	13461	No	1/1	2
18	Yes	smp_001890	194	7900	Yes	0/2/0/2	1
19	Yes	smp_123090	186	7381	Yes	0/2/0/2	1
20	Yes	smp_127130	225	9235	Yes	0/2/0/2	1
21	Yes	smp_159290	234	1582	Yes	0/2/0/2	1
22	Yes	smp_139450	219	1505	Yes	0/2/0/2	1
23	Yes	smp_160250	200	1182	Yes	0/2/0/2	1
24	No	smp_141550	195	1189	Yes	0/2/0/2	1
25	No	smp_141560	195	1189	Yes	0/2/0/2	1
26	Yes	smp_154260	182	2727	Yes	0/2/0/2	1
27	Yes	smp_154290	182	2696	Yes	0/2/0/2	1
28	No	smp_176160	182	2713	Yes	0/2/0/2	1
Genesⁱ encoding incomplete SCP/TAPS domains : smp_120670, smp_116210, smp_176170, smp_035980, smp_159280, smp_100560, smp_118160

(a) Gene names (b) Successful PCR amplification of SmVAL fragment from parasite cDNA (c) Schisto GeneDB version 4 systematic ID (d) Protein size (in amino acids) encoded by open reading frame (e) Gene length (in base pairs) derived from Schisto GeneDB assembly 4 (f) Signal peptide prediction with SignalP 3.0 server (D-score > 0.43 = yes) double asterisk represents SmVALs with D-scores above 0.38 but below 0.43 (g) Conserved Intron Phase derived from Schisto GeneDB assembly 4 (h) Group 1/Group 2 designation. (i) Schisto GeneDB version 4 systematic ID of predicted genes encoding incomplete SCP/TAPS domains are also listed.

ISSN: 1471-2164