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Table 7 Semi-quantitative RT-PCR validation of microarray data.

From: Global gene expression analysis of early response to chemotherapy treatment in ovarian cancer spheroids

Treatment

Gene

Common name

Fold expression

 

p-value

   

Microarray

sqRT-PCR

 

All

BRCA1

breast cancer 1

2,12

1,44

0,0257

All

CASP7

caspase 7

1,53

1,48

0,0625

All

COL25A1

collagen, type XXV, alpha

3,83

1,48

0,0313

All

TLR8

toll-like receptor 8

3,6

1,55

0,0049

All

COX2

cytochrome c oxidase II

1,57

1,31

0,0562

All

SEC8

secretory protein SEC8

-2,02

-1,27

0,0488

cisplatin

GMNN

Geminin

1,97

1,39

0,0612

cisplatin

KCNC1

potassium voltage-gated channel, 1

3,56

1,26

0,0313

cisplatin

LY96

Lymphocyte antigen 96

9,96

1,78

0,0303

cisplatin

TYMS

thymidilate synthase

2,25

1,3

0,0244

topotecan

AHR

aryl hydrocarbon receptor

1,98

1,43

0,0386

topotecan

NR4A1

nuclear receptor subfamily 4, A1

3,17

1,4

0,033

topotecan

S 100A 2

S100 calcium binding protein A2

2,27

1,72

0,0247

topotecan

HLA-E

major histocompatibility complex, IE

-3,02

-1,25

0,0231

paclitaxel

TUBA4

tubulin, alpha

2,59

1,35

0,0247

paclitaxel

TUBB3

tubulin, beta 3

2,51

1,62

0,0135