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Table 2 Composition of Alien® mRNA spike-in mix, 0.5 µl of which was used in every 6-µl cDNA synthesis reaction. Mix1 was added to the Control and the TNT treatment group while Mix2 to the RDX and TNT+RDX groups.

From: Transcriptomic analysis of RDX and TNT interactive sublethal effects in the earthworm Eisenia fetida

Alien spike

Mix1 (ng/µl)

Mix2 (ng/µl)

Ratio Mix1/2

Ratio Mix2/1

AS1

0.1

0.05

2

0.5

AS2

0.05

0.1

0.5

2

AS3

0.01

0.01

1

1

AS4

0.005

0.001

5

0.2

AS5

0.001

0.005

0.2

5