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Table 4 Removal efficiency of mouse and bacterial DNA by different purification methods

From: Evaluation of methods to purify virus-like particles for metagenomic sequencing of intestinal viromes

  FD DTT CsCl MG
Average % read abundance    
Mouse 14.26 10.49 1.20 34.63
Phagea 82.57 86.79 96.13 3.62
Bacteriab 0.033 0.032 0.032 59.21
Calculations based on average % read abundance  
Phage/mouse ratio 5.79 8.27 80.31 0.10
Factor of ratio change compared to MG method 55 79 768 1
Estimated % decrease of read generating mouse DNA as compared to MGc 98.19 98.74 99.87 0.00
Phage/bacteria ratio 2537.74 2674.62 2999.83 0.06
Factor of ratio change compared to MG method 41518 43757 49078 1
Estimated % decrease of read generating bacterial DNA as compared to MGc 99.998 99.998 99.998 0.00
  1. a:Sum of read abundances for all added bacteriophage (this excludes the phiX174 used as Illumina internal control); b:Sum of read abundances for the two added bacteria – L. monocytogenes and B. thetaiotaomicron; c:Calculated based on read abundances normalized to phage read abundance.
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