Integrated transcriptome catalogue and organ-specific profiling of gene expression in fertile garlic (Allium sativum L.)

Table 3 Orthologues of key flowering genes and their relative expression in garlic vegetative and reproductive organs

Flowering genes	Comp number	Relative expression in different organs
Flowering genes	Comp number	Roots	Basal plate	Cloves	Leaves	Inflorescence	Flowers
FLOWERING LOCUS T (FT)	comp149377	0	0	0	0	1.11	3.21
CONSTANS (CO)*	comp45555	0.72	5.99	12.59	5.49	6.87	5.12
SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1)*	comp151688	0	0	0	1.26	1.13	0
LEAFY (LFY)	comp44757	1.61	0	0.77	0	25.35	1.65
APETALA1 (AP1)	comp38587	2.52	62.14	0	50.43	64.89	5.56
APETALA2 (AP2)*	comp46193	3.15	1.45	8.64	0.98	0.54	22.46
APETALA3 (AP3)	comp35626	0	0	0.57	0	40.93	245.92
PISTILLATA (PI)	comp44196	0.56	0.55	0.27	0	2.58	143.66
SEPALLATA1 (SEP1)*	comp56369	0	0.93	0	0	86.97	13.48
SEPALLATA3 (SEP3)	comp42485	0.52	0	0	0	26.16	263.92
AGAMOUS (AG)*	comp59363	0.9	0	0	0	4.83	185.44

Sequences of known flowering genes from public databases were matched with the garlic abundant transcriptome. The expression level (FPKM) was calculated using the expectation maximization method, and TMM normalization (trimmed mean of M-value normalization method) was applied (see Materials and Methods).
*Numerous sequences with significant similarity were identified.

ISSN: 1471-2164