Table 1 List of putative reference genes and genes of interest

R1

DUF221 domain protein (DUF221)

Vacuolar membrane (GO:0005774)

Transmembrane protein with unknown function

Fw: CGGAACGCCCCATTGACC

95.1%

126 bp

Rv: TTGGATGCTTATGTTTTGCTCTCG

R2

Ubiquitin carrier protein (ubcB)

Ligase activity (GO:0016874)

Involved in the ubiquitin mediated proteolysis

Fw: TCGTTGAGTAGACTCTGAATGCTG

99.2%

125 bp

Cellular response to stress (GO:0033554)

Rv: AGCCAGATGTTCCACCCG

Cytoplasm (GO:0005737)

R3

CECR1 family adenosine deaminase (ADA)

Adenosine deaminase activity (GO:0004000)

Involved in the purin metabolism

Fw: CTGCGCAATGCAAAGTCATGTCTCTG

100.7%

97 bp

Rv: CCCAGGTCGAAGATCCCTTTATCCA

R4

Mitochondrial membrane fission protein (fis1)

Metal ion binding (GO:0046872)

Mitochondrial complex that promotes mitochondrial fission

Fw: GTTCAACTACGCCTGGGGACTC

101.1%

91 bp

Mitochondrial fission (GO:0000266)

Rv: AGCGGTGCGAAAAATCTGGG

Membrane (GO:0016020)

R5

Copper-transporting ATPase (Cu-ATPase)

Nucleotide binding (GO:0000166)

Fw: TGGTGCCCTGTGCCAACTCTCCCAGTC

103.6%

78 bp

Cellular metal ion homeostasis (GO:0006875)

Rv: TTGCTGCGGGTGCTTTTG

Membrane (GO:0016020)

R6

Cohesin complex subunit (psm1)

DNA secondary structure binding (GO:0000217)

Involved in chromosomes segregation during mitosis

Fw: GTATTTGCGGAGATCCAGAGTGAG

93.1%

102 bp

Mitotic sister chromatid segregation (GO:0000070)

Rv: TTGAAGACGGGTCTGTTCCA

Nucleus (GO:0005634)

R7

Spo7-like protein (spo7)

Phosphatase activity (GO:0016791)

Involved in the spore formation process

Fw: GCCGATGGTGCTGATGTTGG

102.5%

110 bp

Sporulation (GO:0043934)

Rv: AGAACGCCAACGAGCCCG

Integral to membrane (GO:0016021)

R8

SAGA-like transcriptional regulatory complex subunit Spt3 (spt3)

Transferase activity (GO:0016740)

Component of the nuclosomal histone acetyltransferase (SAGA) complex

Fw: ACGACTTGTTGGCGGACG

96.3%

95 bp

Chromatin modification (GO:0016568)

Rv: GAGATTCAGCAGATGATGTTTGTC

Nucleus (GO:0005634)

R9

DUF500 domain protein (DUF500)

Actin filament organization (GO:0007015)

Cytoskeleton organization

Fw: ACTTGGCCGGTTGTGCGTTC

98.5%

101 bp

Cytoplasm (GO:0005737)

Rv: TTGGTGTTCCGGCGGCTG

R10

Rho GTPase activator (sac7)

Rho GTPase activator activity (GO:0005100)

Involved in signal transduction

Fw: AGGAGGATGAAAGTAAAGGACCCC

100.5%

159 bp

Small GTPase mediated signal transduction (GO:0007264)

Rv: AAACCCCACACTTGGCGAC

Intracellular (GO:0005622)

R11

AP-2 adaptor complex subunit beta (AP-2 β)

Transporter activity (GO:0005215)

Involved in chlatrin-dependent endocytosis

Fw: TTTCGCACATAGGGGTCG

98.4%

148 bp

Rv: TTTTGGTCGATGATATGGACG

R12

Protein translocation complex componenet (npl1)

Protein transporter activity (GO:0008565)

Involved in the protein progression in endoplasmic reticulum

Fw: CGCTGGAACAAGAAAAATACG

98.2%

117 bp

Post-translational protein targeting to membrane (GO:0006620)

Rv: ACGAACGATATGCGCCAA

Endoplasmic reticulum (GO:0005783)

β-tub

Beta-tubulin

Nucleotide binding (GO:0000166)

Cytoskeleton

Fw: GTTCTGGACGTTGCGCATCTG

97.2%

110 bp

Cytoskeleton (GO:0005856)

Rv: TGATGGCCGCTTCTGACTTCC

abf-B2*

Arabinofuranosidase-B2

Hydrolase activity, acting on glycosyl bonds (GO:0016798)

Sugar metabolism

Fw: CGGAGCTTGGGTGAGATGGTTC

103.6%

112 bp

Carbohydrate metabolic process (GO:0005975)

Rv: CGGCGGCGTTGCTAATGC

Extracellular region (GO:0005576)

xynC*

Xylanase C

Hydrolase activity, acting on glycosyl bonds (GO:0016798)

Sugar metabolism

Fw: CAAATGGCGACAATGGCG

94.4%

104 bp

Xylan metabolic process (GO:0045493)

Rv: TGAGTACGTGACAGTCTGTGCATTG

Extracellular region (GO:0005576)