Treatment with TIIA changes the expression of glucose metabolism-related proteins in AGS cells. (A) The expression of iTRAQ-identified proteins, LDHB and ENO1 was estimated using western blotting. (B) The expression of ALDOC, MDH1, PCK2 and PGK1 in AGS cells treated with TIIA was estimated using western blotting. The levels of (C) tumor suppressor gene, p53, and the oncogene AKT, and (D) intracellular ATP, were examined in AGS cells treated with TIIA. β-actin was used as an internal control.