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Table 2 Comparison between the values for fold change of some genes using RNA-Seq and qPCR

From: Next generation sequencing analysis reveals that the ribonucleases RNase II, RNase R and PNPase affect bacterial motility and biofilm formation in E. coli

Transcript

Mutant

RNA-Seq 1

qPCR 1

Ag43 ( flu )

Δrnb

10.3

12.9

 

Δrnr

1.1

1.2

 

Δpnp

7.8

11.2

bssR

Δrnb

0.6

0.6

 

Δrnr

5.7

1.6

 

Δpnp

0.2

0.4

flhC

Δrnb

0.4

0.8

 

Δrnr

0.3

0.4

 

Δpnp

1.4

2.6

flgJ

Δrnb

0.2

0.4

 

Δrnr

0.9

0.6

 

Δpnp

0.4

0.6

fliH

Δrnb

0.2

0.2

 

Δrnr

0.8

0.5

 

Δpnp

0.3

0.6

fliA

Δrnb

0.2

0.2

 

Δrnr

0.7

0.6

 

Δpnp

0.9

0.8

  1. 1Fold Changes were calculated as the ratio of mutant to WT. Values above 1 correspond to up-regulated transcripts while values below 1 correspond to down-regulated transcripts.
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BMC Genomics

ISSN: 1471-2164

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