Transcriptome analysis of thermophilic methylotrophic Bacillus methanolicus MGA3 using RNA-sequencing provides detailed insights into its previously uncharted transcriptional landscape

Table 2 Largest identified primary operons on the B. methanolicus MGA3 chromosome

Genes	No. of genes	Strand	Putative gene functions	Transcriptional organization in *B. subtilis* ¹
BMMGA3_00690-BMMGA3_00840	31	+	Various, mainly translation and ribosomal structure	Similar, [31]
BMMGA3_06100-BMMGA3_06250	31	+	Movement and chemotaxis	Similar, [29]
BMMGA3_01635-BMMGA3_01690	12	+	Purine biosynthesis	Similar, [32]
BMMGA3_15075-BMMGA3_15130	12	-	Amino sugars biosynthesis and polymerization	No similar operon present
BMMGA3_05780-BMMGA3_05825	10	+	Pyrimidine biosynthesis	Similar, [33]
BMMGA3_15975-BMMGA3_16020	10	-	ATP synthesis	[8/10] + 1, [34]
BMMGA3_12730- BMMGA3_12770	9	-	Biosynthesis of branched amino acids	[8/9] + 1, [35]
BMMGA3_01305-BMMGA3_01345	9	+	Control of SigB activity, general stress response	[7/9] + 1, [36]
BMMGA3_02555-BMMGA3_02595	9	+	Sulfate reduction and activation, siroheme biosynthesis	[8/9]* + 2, [37]
BMMGA3_00415-BMMGA3_00450	8	+	Folic acid biosynthesis	[7/9]^§ [38]
BMMGA3_05870-BMMGA3_05905	8	+	Various, mainly translation, ribosomal structure, signal transduction	[3 + 3/7]^‡ +2, [39,40]
BMMGA3_14725-BMMGA3_14760	8	-	Histidine biosynthesis	Similar, [41]

¹ Genes in the same transcriptional organization as B. methanolicus MGA3 are depicted in square brackets. The number of transcripts that are present in the B. subtilis genome, but are not associated to the respective operon are indicated after the plus sign.
* In comparison to the B. methanolicus MGA3 the gene order is altered.
^§ In B. subtilis, the operon contains one additional gene.
^‡ In B. subtilis, the genes are organized in two separate operons with.

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