Transcriptome analysis of thermophilic methylotrophic Bacillus methanolicus MGA3 using RNA-sequencing provides detailed insights into its previously uncharted transcriptional landscape

Table 3 Novel transcripts with known function identified in B. methanolicus MGA3

Name	Locus tag	Class	Feature start	Feature stop	Length	Strand
4.5S RNA/scRNA	BMMGA3_s0190	sRNA	27,291	27,628	307	+
RNase P RNA	BMMGA3_s0200	sRNA	2,087,761	2,087,336	425	-
6S RNA/SsrS	BMMGA3_s0210	sRNA	2,278,029	2,277,818	211	-
6S RNA/SsrS	BMMGA3_s0220	sRNA	2,437,070	2,436,840	230	-
tmRNA/SsrA	BMMGA3_s0230	sRNA	2,890,631	2,890,214	417	-
SR4 RNA	BMMGA3_s0240	sRNA	1,925,025	1,924,851*	174	-
bsrG	BMMGA3_9642	Protein	1,924,777	1,924,908	132	+

*The stop of this feature was approximated based on the published sequence in B. subtilis [44] and the terminator predication with ARNold [45].

ISSN: 1471-2164