Figure 6

The effect of Htz1 on antisense transcript levels impacts sense transcripts. A. Genes with 3' Htz1 enrichment and an antisense transcript were divided into three groups based on whether their antisense transcript levels were unchanged (n = 794), up- (n = 28) or down- (n = 159) regulated in rrp6∆htz1∆. The boxplots show changes in sense (S) transcript levels for these groups. Down-regulated antisense transcripts have a significantly higher fold change in sense expression (*p ≤ 0.05 (0.02), two-tailed t-test) and conversely sense transcript levels are significantly decreased when antisense transcripts are up-regulated (*p ≤ 0.05 (0.05), two-tailed t-test). B. Classification of genes with both sense and antisense transcripts according to 5' and 3' enrichment of Htz1 was performed using thresholds for 5' and 3' enrichment based on average Htz1 occupancies at the 5' and 3' ends. Class 1 (n = 110) have only 5' enrichment; class 2 (n = 237) have 5' and 3' enrichment; class 3 (n = 75) are not enriched for Htz1 at either 5' or 3' ends; and class 4 (n = 34) have Htz1 only at the 3' end. C. Distributions of sense transcript fold changes in the rrp6∆htz1∆ strain for each of the classes of genes illustrated in B. Sense transcripts are significantly up-regulated in group 4 genes that have Htz1 at their 3' ends only (***p ≤ 0.001 (7.5 x 10⁻⁴), two-tailed t-test).