Figure 4

Expression of neuronal marker genes and genes associated with Alzheimer-related brain regions in AD-iPS neurons. (a) Cluster analysis of neuronal marker genes in AD-iPS neurons and H9 neurons based on Euclidean distance of microarray-based gene expression values. (b) A number of genes of the heatmap data in (a) were confirmed by real- time PCR analysis. Bars indicate the RNA level normalized to β-ACTIN first and compared to gene expression of adult brain tissue (non-diseased, male, 21 years; Amsbio). The samples of AD-iPS neurons and H9 neurons consisted of cRNA generated from RNA isolated from a single well of a single neuronal differentiation respectively. In addition, to our data we analyzed AD brain RNA (male, 87 years, diagnosed AD; Amsbio). (c) Brain regions associated with genes from the AD KEGG pathway (but neither in Huntington disease nor in Parkinson disease) down-regulated in AD-iPS5 compared to H9 neurons. (d) Brain regions associated with genes from the AD KEGG pathway (but neither in Huntington nor in Parkinson disease) down-regulated in AD-iPS26B compared to H9 neurons.