Experimental validation of 3 strain-specific splicing events. PCR validation, using striatum cDNA, of 3 SSS events. SSS events found within Hddc3
(B), and Atf6
(C) are depicted in purple. The B6 SSS event found within the Hddc3 gene is supported with a read coverage of 20 and results in the use of an alternative last exon. The B6 SSS event found within the Gabra2 gene is supported with a read coverage of 6 and results in a skipping of an exon. The CAST SSS event found within the Atf6 gene is also supported by 6 reads. PCR products confirming each SSS event are indicated with purple arrows. A splicing event conserved within all 8 strains (blue), within each gene, served as a positive control. Blue arrows indicate the PCR products confirming each conserved splicing event. The length of the expected PCR product(s) from each forward (F) and reverse (R) primer are indicated. Water served as a negative control. A 100 bp ladder was loaded to confirm PCR product sizes.