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Table 3 Regression coefficients of C t values on dilution factors

From: Simple regression for correcting ΔCt bias in RT-qPCR low-density array data normalization

Samples

RPS9

GAPDH

RPL13A

IFNGR1

IRF1

LY96

X5028

−1.133

−1.224

−1.062

−1.541

−1.082

−1.249

X5030

−1.078

−1.229

−1.072

−1.63

−1.081

−1.194

X7057

−1.094

−1.218

−1.088

−1.583

−1.072

−1.145

X7062

−1.09

−1.229

−1.081

−1.621

−1.091

−1.189

  1. Dilution factors were log2 transformed. The ideal 100% efficiency corresponds to coefficient of −1.