Skip to main content

Table 3 Regression coefficients of C t values on dilution factors

From: Simple regression for correcting ΔCt bias in RT-qPCR low-density array data normalization

Samples RPS9 GAPDH RPL13A IFNGR1 IRF1 LY96
X5028 −1.133 −1.224 −1.062 −1.541 −1.082 −1.249
X5030 −1.078 −1.229 −1.072 −1.63 −1.081 −1.194
X7057 −1.094 −1.218 −1.088 −1.583 −1.072 −1.145
X7062 −1.09 −1.229 −1.081 −1.621 −1.091 −1.189
  1. Dilution factors were log2 transformed. The ideal 100% efficiency corresponds to coefficient of −1.