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Figure 2 | BMC Genomics

Figure 2

From: Genome analysis of Flaviramulus ichthyoenteri Th78T in the family Flavobacteriaceae: insights into its quorum quenching property and potential roles in fish intestine

Figure 2

Expression of FiaL and detection of its QQ activity. (A). SDS-PAGE analysis of overexpressed FiaL. Lane 1, protein molecular weight marker (Fermentas SM0431); Lane 2, supernatant of E. coli/pET 24a(+) after ultrasonication; Lane 3, resuspended cell pellet of E. coli/pET 24a(+) after ultrasonication; Lane 4, supernatant of E. coli/pET 24a(+)/GL001211 after ultrasonication; Lane 5, resuspended cell pellet of E. coli/pET 24a(+)/GL001211 after ultrasonication (the arrow indicates the position of FiaL). (B). AHL-degrading activity of recombinant E. coli BL21(DE3). 1, supernatant of E. coli/pET 24a(+)/GL001211 after ultrasonication; 2, supernatant of E. coli/pET 24a(+) after ultrasonication.

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BMC Genomics

ISSN: 1471-2164

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