Figure 1

miR-503, −103, and −494 inhibit proliferation. (A) miR-503, −103, and −494 transfection significantly reduces primary human fibroblast cell growth. Average cell number relative to 0 hr following miRNA or Control siRNA transfection is shown for each time point indicated. Error bars denote ± SD, n = 6. P-values were calculated by Student’s t-test comparing cell numbers following miRNA transfection to cell numbers following Control siRNA transfection at each time point. (B) miR-503 and −494 transfection significantly represses proliferation. The Y-axis indicates the relative percentage of primary human fibroblast cells expressing Ki67, measured by flow cytometry. Bars are the mean percentage of cells expressing Ki67 relative to Control RNA 2, and error bars denote ± SD, n = 3. (C) miR-503 and −103 transfection significantly inhibits progression through the cell cycle. The Y-axis indicates the percentage of primary human fibroblast cells found in each stage of the cell cycle measured using propidium iodide staining, and bars are the mean percentage the cell population found in each stage. Error bars denote ± SD, n = 3. (D) Inhibition of miR-503, −103, and −494 increases cell growth in quiescent fibroblasts. Average cell number relative to 0 hr following transfection of an LNA targeting miR-503, −103, or −494 or a LNA negative control is shown for each time point indicated. Error bars denote ± SD, n = 6. P-values were calculated by Student’s one tailed t-test comparing cell numbers following miRNA inhibitor transfection to cell numbers following Control inhibitor transfection at each time point. For B, P-values were estimated by Student’s t-test, and for C, P-values were estimated by Student’s paired t-test. ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05.