Figure 7From: Transcriptomic analysis of hepatic responses to testosterone deficiency in miniature pigs fed a high-cholesterol dietExpression of genes involved in immune and inflammatory responses. (A) Heat map for genes involved in immune and inflammatory responses. (B) Expression levels of six genes detected by quantitative real-time RT-PCR (yellow box) were in agreement with the RNA-Seq results. Data are expressed as means ± SEMs, n = 6 per group. (C) Immunohistochemical assessment of chemokine (C-C motif) ligand 2 (CCL2), chemokine (C-X-C motif) ligand 9 (CXCL9), and interferon-regulatory factor 7 (IRF7), showing expression changes that were in accordance with the RNA-Seq and qRT-PCR results. IM: intact male pigs fed an HFC diet; CM: castrated male pigs fed an HFC diet; CMT: castrated male pigs fed an HFC diet and given testosterone replacement therapy. Scale bars = 100 μm.Back to article page