Expression of genes involved in immune and inflammatory responses. (A) Heat map for genes involved in immune and inflammatory responses. (B) Expression levels of six genes detected by quantitative real-time RT-PCR (yellow box) were in agreement with the RNA-Seq results. Data are expressed as means ± SEMs, n = 6 per group. (C) Immunohistochemical assessment of chemokine (C-C motif) ligand 2 (CCL2), chemokine (C-X-C motif) ligand 9 (CXCL9), and interferon-regulatory factor 7 (IRF7), showing expression changes that were in accordance with the RNA-Seq and qRT-PCR results. IM: intact male pigs fed an HFC diet; CM: castrated male pigs fed an HFC diet; CMT: castrated male pigs fed an HFC diet and given testosterone replacement therapy. Scale bars = 100 μm.