Figure 8

Expression of genes involved in fatty acid oxidation and cholesterol, bile acid, and glucose metabolism. (A) Heat map for genes involved in fatty acid oxidation. (B) Heat map for genes involved in cholesterol and bile acid metabolism. (C) Heat map for genes involved in glucose metabolism. (D, E) Expression levels of six genes detected by quantitative real-time RT-PCR (qRT-PCR) that were in agreement with the RNA-Seq results. Data are expressed as means ± SEMs, n = 6 per group. (F, G) Western blot analysis of peroxisome proliferator-activated receptor delta (PPARD) and carnitine palmitoyltransferase 1A (CPT-1A) showing expression changes that were in accordance with RNA-Seq and qRT-PCR results. IM: intact male pigs fed an HFC diet; CM: castrated male pigs fed an HFC diet; CMT: castrated male pigs fed an HFC diet and given testosterone replacement therapy. Data are expressed as means ± SEMs, n = 5 per group. *P < 0.05.