Figure 1

Introduction of synonymous tracer mutations in gene segment 7 of PR8 virus. (A) Schematic representation of the influenza M segment present in pHW197-Mmut. The open reading frames of M1 (yellow, starting at position 489, relative to the upstream CMV promoter (not depicted)) and M2 (orange, starting at position 489 and ending at position 1470) are indicated. The resulting HindIII and PvuII restriction sites are indicated. (B) Fluorograms showing the synonymous substitutions in pHW197-Mmut relative to pHW197-M at positions 797 (C to T) and 1088 (A to T). The predicted amino acid sequence is shown underneath the nucleotide sequence.