Figure 10

Low frequency minor alleles are detected at significantly higher frequencies by Illumina MiSeq compared to Ion Torrent PGM. Nucleotide variants were subdivided in two frequency classes: high (frequency minor allele > 15%, n = 4) and low (frequency minor allele: < 15%, n = 42). Mean proportions ± s.e. of the minor variants detected in PR8 and PR8mut viral samples by the Illumina MiSeq and Ion Torrent PGM are shown. Minor allele proportions were analyzed by logistic regression (link function = logit). Significance levels of pairwise comparisons were assessed by a Fisher’s protected least significance difference test * = p < 0.05, ** = p < 0.01.