Figure 9
From: Analysis of the genetic diversity of influenza A viruses using next-generation DNA sequencing
Coverage of PR8 virus genome with the optimized RT-PCR protocol. Sequence coverage for the different genome segments of wild type PR8 virus sequenced on Illumina MiSeq (2x250 bp) using two different fragmentation methods: Nextera XT transposase-based fragmentation (black lines) and mechanical Covaris shearing followed by adaptor ligation (orange lines). The obtained sequences were mapped to the reference genome (based on the plasmids used to generate the virus).