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Table 4 Percent coverage of the influenza PR8 reference sequence after de novo assembly

From: Analysis of the genetic diversity of influenza A viruses using next-generation DNA sequencing

Segment

Illumina MiSeq a (SD b )

Ion torrent PGM a (SD b )

PB2

99.55 (0.30)

100.00 (0.00)

PB1

99.37 (0.52)

100.00 (0.00)

PA

99.35 (0.54)

99.30 (0.21)

HA

98.65 (0.52)

99.04 (0.50)

NP

98.79 (0.92)

98.07 (0.00)

NA

98.92 (0.82)

99.97 (0.07)

M - Mmut

98.20 (1.36)

99.55 (0.89)

NS

96.94 (0.76)

98.17 (2.12)

  1. aViral RT-PCR product sequencing reads obtained on Illumina MiSeq and Ion Torrent PGM were de novo assembled, followed by alignment of the obtained consensus sequence to the PR8 (n = 2) or PR8mut (n = 2) reference genome. For each segment, the percentage of the influenza reference sequence (based on the sequence from the plasmids from which the virus was produced) that is covered by the assembled contigs is given.
  2. bSD = standard deviation.
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BMC Genomics

ISSN: 1471-2164

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