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Table 7 Number of variants detected in wild type and mutant PR8 quasispecies after filtering

From: Analysis of the genetic diversity of influenza A viruses using next-generation DNA sequencing

  

Illumina MiSeq

Ion Torrent PGM

Shared

  

S1 a

S2 a

shared

S1 a

S2 a

Shared

PR8

SNP b

25

26

24

19

21

18

18

Insertion

0

0

0

1

1

0

0

Deletion

6

5

4

9

9

3

1

PR8mut

SNP b

48

46

46

32

37

32

29

Insertion

0

0

0

4

4

4

0

Deletion

5

6

5

8

11

4

0

  1. The filtering parameters were: average quality threshold > Q20, forward/reverse balance > 0.25, independent counts of variant > 10, and frequency > 0.5%.
  2. aThe wild type and mutant PR8 quasispecies were sequenced in duplicate (S1 and S2). bSNP = single nucleotide polymorphism.
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BMC Genomics

ISSN: 1471-2164

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