Figure 6

Electrocyte-specific microRNA expression. (a) Heatmap of miRNA expression in E. electricus and S. macrurus. Values are log₂-transformed and median-centered values of tissue-specific expression for each miRNA, such that blue indicates under-expression and red indicates over-expression relative to the median. The miRNAs shown are limited to those with >4-fold increased or decreased expression in E. electricus electric organs compared to skeletal muscle. Log₂ values are clamped between −3 and +3. Asterisks indicate novel E. electricus miRNAs. (b) Schematic diagram of the kcnj12b gene locus and novel electro-miR mir-11054 on scaffold5041 of the E. electricus genome. Thin boxes are UTRs, the thick box with white directional arrows is the coding sequence, and the thin line is an intron. The red arrow on the antisense strand indicates the location of the novel mir-11054 microRNA.