Figure 6

Analyses of common genes and their biological function between iAs-T and iAs-Rev cells. A) Venn diagram analyses showing the genes common between the two conditions. B) We used 100% stacked column analysis to compare the changes and biological functions of genes under arsenic treatment and in reversed conditions. Bar graph representing the percentage of genes and their biological process altered in iAs-T and after iAs is withdrawn from iAs-T, referred to as iAs-Rev cells. C) Identification of unique and shared genes between iAs-T and genes that did not revert after arsenic is withdrawn. The Venn diagram indicates the number of unique and common differentially expressed genes at each treatment condition. D) Heatmap analysis of gene expression changes from microarray data in iAs-T and iAs-Rev cells. The analysis shows relative fluorescence from green (downregulated) to red (upregulated) of the six genes found common in the experimental conditions. qRT-PCR analyses to validate the microarray data in E) HeLa cells and F) Beas-2B cells.