Figure 7
Immunoblot analysis of iAs-target genes in both HeLa and BEAS-2B cells. A) Shown are representative immunoblots with expanded views of antibody-decorated bands. Immuno-decoration was carried out with primary antibodies against the respective proteins CD36, GDF15, HO-1. Besides the individual immunoblots are shown panels, which give a graphical representation of the immuno-decoration levels of the various proteins (Student’s t-test p < 0.05). B) MNase-treated DNA from cells from all experimental conditions was subjected to quantitaitive real-time PCR analysis using primers that map to the promoters of the respective genes. Note that changes in nucleosome occupancy correlates with the gene expression patterns of the various genes in the different experimental conditions. The average results ± SD from two independent experiments are shown.