Table 2 MiRNA recovery by kit, presented as average Cq ± SD (n = 10)
From: Optimized microRNA purification from TRIzol-treated plasma
RNA Target | Ambion miR vana miRNA Isolation Kit | Exiqon RNA Isolation Kit -Biofluids | QIAGEN RNeasy Mini Kit | QIAGEN miRNeasy Mini Kit | Zymo Direct-zol RNA MiniPrep Kit |
|---|---|---|---|---|---|
UniSp2 | 28.6 ± 0.3 | 24.9 ± 0.4 | 21.8 ± 0.4 | 21.5 ± 0.3 | 23.7 ± 0.5 |
UniSp4 | 34.8 ± 0.9 | 31.9 ± 0.6 | 28.9 ± 0.8 | 28.7 ± 0.8 | 30.9 ± 0.4 |
UniSp5 | 33.7 ± 6.0(3) | 36.4 ± 0.4(7) | 34.7 ± 1.4 | 34.1 ± 2.0 | 38.3 ± 3.6(8) |
hsa-miR-451a | 30.6 ± 0.4 | 26.8 ± 0.5 | 23.7 ± 0.3 | 23.6 ± 0.4 | 25.2 ± 0.5 |
hsa-miR-16-5p | 31.3 ± 0.4 | 27.6 ± 0.5 | 24.7 ± 0.4 | 24.8 ± 0.5 | 26.6 ± 0.5 |
hsa-miR-93-5p | 34.6 ± 0.5 | 31.0 ± 0.5 | 28.4 ± 0.4 | 28.5 ± 0.4 | 30.3 ± 0.5 |
hsa-miR-23a-3p | 35.8 ± 0.6 | 31.9 ± 0.4 | 29.6 ± 0.4 | 29.5 ± 0.5 | 31.3 ± 0.6 |
hsa-miR-423-3p | 40.6 ± 4.7(4) | 33.1 ± 0.8 | 31.1 ± 0.4 | 31.1 ± 0.4 | 33.2 ± 0.4 |
hsa-miR-103a-3p | 38.9 ± 1.5(8) | 33.9 ± 0.5 | 31.5 ± 0.3 | 31.5 ± 0.5 | 32.2 ± 1.2 |
hsa-miR-205-5p | NA(0) | 36.5 ± 0.5(9) | 35.5 ± 0.6 | 35.6 ± 0.6 | 38.1 ± 1.9 |