Figure 1

ChIP sequencing of PU.1 and Spi-B in a mouse B lymphoma cell line. A. Workflow for generating ChIP sequencing data. B. Venn diagram for regions of significant ChIP binding determined by MM-ChIP. Regions with at least 100 base pair overlap were considered common to both factors. C. Venn diagram of peakset regions analyzed in DiffBind. The majority of regions in the matrix demonstrated less than 2 fold difference in enrichment between PU.1 and Spi-B. D. Scatter plot of ChIP signal between PU.1 and Spi-B binding. Binding of these transcription factors was similar across most of the genome. E. De-novo motif analysis from ChIP. From extracted DNA sequences, frequently occurring motifs were discovered using MEME-ChIP. The most frequently occurring motif for each sample contained the canonical ETS binding sequence F. Representative ChIP-seq binding at a region considered to be regulated by PU.1 and Spi-B. The histocompatibility 2 Q region on chromosome 17 demonstrates high peaks for PU.1 and Spi-B at gene promoters.