Figure 4

SigR-dependent transcription activation of the ndgR gene in S. coelicolor. (A) Quantitation of ndgR transcripts using quantitative realtime-PCR analysis (qRT-PCR) from diamide-treated cells reveals that the ndgR gene is induced using the SigR promoter. mRNA level of ndgR in WT (black bars) was induced under diamide treatment compared to the level of ndgR in sigRrsrA deletion mutant (gray bars). All levels are normalized by the levels of each sample at 0 min. (B) Sensitivity test of WT, ndgR deletion mutant (BG11) and complemented mutant (BG13) under thiol oxidative stress. Serially diluted spores of WT, BG11 and BG13 were spotted on R5 agar plates with or without added diamide (0.6 mM). Plates were incubated at 30°C for 5 days.