Figure 6

DNase activity of the recombinant protein Sjda-GST. Agarose gel electrophoresis was carried out to analyse the catalytic products of the DNase. A. The left panels show the DNA digested by 40 μg Sjda-GST or GST proteins at the indicated times. The right panels show the digested DNA according to the indicated amount of Sjda-GST or GST proteins with an incubation time of 15 min. All assays were carried out with 200 ng human genomic DNA in a final volume of 25μlPBS (pH7.0) at 37°C. B. The DNA from different hosts digested by Sjda-GST or GST. H, human; C, calf; R, rabbit; and M, C57BL/6 mouse. C. S. japonicum DNA digested by Sjda-GST or GST. D. Human DNA digested by Sjda-GST in PBS of different pH levels, ranging from 4.0 to 7.5. E. Supercoiled plasmid DNA digested by Sjda-GST, GST or XhlI.