Identification of three extra-chromosomal replicons in Leptospira pathogenic strain and development of new shuttle vectors

Table 1 Transformation efficiency of shuttle vectors constructed in this study in four Leptospira strains

Leptospira strains	lcp1L/S	lcp2L/S	lcp3L/S	pGKBLe24 ^-rep			ddH ₂ O	pGKBLe24
Leptospira strains	lcp1L/S	lcp2L/S	lcp3L/S	lcp1	lcp2	lcp3	ddH ₂ O	pGKBLe24
L. interrogans serovar Autumnalis str. 56606	-	-	+	-	-	-	-	-
L. interrogans serovar Hebdomadis str. 56610	+	-	+	-	-	-	-	-
L. interrogans serovar Lai str. 56601	+	-	+	-	-	-	-	-
L. biflexa serovar Patoc str. Patoc I	-	+	+	-	-	-	-	+
L. interrogans serovar Linhai str. 56609	-	-	-	-	-	-	-	-

ISSN: 1471-2164