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Figure 2 | BMC Genomics

Figure 2

From: Comparative analysis among the small RNA populations of source, sink and conductive tissues in two different plant-virus pathosystems

Figure 2

Analysis of the total sRNA population on silver nitrate-stained polyacrylamide gels and isotopic detection of the viral sRNAs (vsRNAs) by Northern blot. (A, B and C upper pannels) Separation of the total sRNAs in polyacrylamide/Urea gels stained with silver nitrate. (A) Melon tissues; healthy leaf, cotyledon and root (lanes 1, 2 and 3) and MNSV-infected leaf, cotyledon and root (lanes 4, 5 and 6). (B) Cucumber tissues; healthy leaf, cotyledon and root (lanes 7, 8 and 9) and PNRSV-infected leaf, cotyledon and root (lanes 10, 11 and 12). (C) Melon (lane 13) and cucumber (lane 15) healthy phloem, MNSV (lane 14) and PNRSV (lane 16) infected phloem. (A, B and C lower pannels) Northern blot analysis to detect MNSV (A y C) and PNRSV (B y C) derived sRNAs in the different tissues. (D). Detection limit of the PNRSV probe. Serial dilutions of an artificial PNRSV sRNA were blotted onto a nylon membrane and hybridized with the isotopic PNRSV probe. LC: Loading control.

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BMC Genomics

ISSN: 1471-2164

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