Figure 7

Validation of MNSV hot-spots by stem-loop RT-PCR. (A) Schematic representation of the complete genome of MNSV with the different open reading frames indicated by colors. Numbers represent the 5′position on the genome of the three selected hot-spots and their polarity is shown between brackets. (B) Stem-loop RT-PCR analysis of the three hot-spots in all the tissues. Healthy leaf (1), MNSV infected leaf (2), healthy cotyledon (3), MNSV infected cotyledon, healthy root (5), MNSV infected root (6), healthy phloem (7) and MNSV infected phloem (8). As a positive and negative controls in phloem, miR159 and miR171 respectively were used.