Figure 8

Validation of PNRSV hot-spots by stem-loop RT-PCR. (A) Schematic representation of the RNA3 of PNRSV. Numbers represent the 5′position on the genome of the two selected hot-spots and their polarity between brackets. (B) Stem-loop RT-PCR analysis of the three hot-spots in all the tissues. Healthy leaf (1), PNRSV infected leaf (2), healthy cotyledon (3), PNRSV infected cotyledon (4), healthy root (5), PNRSV infected root (6), healthy phloem (7) and PNRSV infected phloem (8). As positive and negative controls in phloem, miR159 and miR171 respectively, were used.