Figure 4

Maximum parsimony tree of Guatemalan B2 mitogenomes. The position of the rCRS is indicated for reading off sequence motifs. Mutational changes are shown along branches (in blue are those falling in the control region); mutations are transitions unless a suffix A, C, G, or T indicates a transversion. Other possible suffixes are: insertions (+), synonymous substitutions (s), mutations changes occurring at tRNAs (−t), mutational changes occurring at rRNAs (−r), non-coding variants located in the mtDNA coding region (−nc), and amino acid replacements indicated in round brackets. A back mutation is represented with the prefix “@” (a double “@” would indicate a double recurrent mutation), whereas an underlined mutation represents a recurrent mutation in the phylogeny represented in the figure. As usual, variants at positions A16182C, A16183C, variation around position 310 and length or point heteroplasmies were not considered for the phylogenetic reconstruction. Maximum likelihood (ML) coalescence ages (Table 1) are indicated in the top left corner of each haplogroup label (black background boxes). Green boxes containing haplogroup labels indicate new clades determined in the present study with respect to Phylotree. Coding region segments were not used for estimates of the TMRCA.