Characterization and expression profiling of glutathione S-transferases in the diamondback moth, Plutella xylostella (L.)

Table 1 Description of 22 identified cytosolic GSTs in the P. xylostella genome

Gene name	ORF (bp)	Protein (AA)	Gene size (bp)	Scaffold/orientation	Gene ID ^a
PxGSTd1 ^b	654	217	2534	38/+	Px010343
PxGSTd2	648	215	3031	75/+	Px015896
PxGSTd3^b	660	219	1640	75/-	Px015897
PxGSTd4	672	223	2321	221/+	Px006286
PxGSTd5	672	223	2294	73/-	Px015631
PxGSTe1	699	232	1823	66/+	Px014816
PxGSTe2 ^b	684	227	3497	363/-	Px010078
PxGSTe3	687	228	1699	41/+	Px011036
PxGSTe4	663	220	2922	216/+	Px006106
PxGSTe5	651	216	4863	216/+	Px006105
PxGSTo1	768	255	2649	85/+	Px016897
PxGSTo2 ^b	750	249	6717	554/+	Px016898
PxGSTo3	726	241	3721	25/+	Px007118
PxGSTo4	750	249	750	7/-	Px015266
PxGSTo5	738	245	2200	554/+	Px013473
PxGSTs1	615	204	2669	320/-	Px009113
PxGSTs2	615	204	8124	328/-	Px009257
PxGSTt1	654	217	3261	547/-	Px000759
PxGSTu1 ^b	693	230	2771	1088/-	Px000790
PxGSTu2	648	215	963	408/-	Px010993
PxGSTz1	645	214	2450	16/-	Px003659
PxGSTz2	642	213	15681	115/+	Px001225

^aThe gene IDs were obtained directly from the published DBM genomic database (DBM-DB: http://iae.fafu.edu.cn/DBM/family/PxGSTs.php). All the coding sequences (CDS) of the PxGST genes have been experimentally validated.
^bCoding sequences of such genes were incomplete from the DBM-DB, and have been experimentally completed by PCR, as explained in the methodology.
Px: Plutella xylostella.

ISSN: 1471-2164