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Figure 1 | BMC Genomics

Figure 1

From: TILLING by sequencing to identify induced mutations in stress resistance genes of peanut (Arachis hypogaea)

Figure 1

PCR amplicons of AhLOX7 _5', AhLOX7_3 ', AhPLD1 , and AhPLD2. PCR amplification of AhLOX7_5', AhLOX7_3', AhPLD1, and AhPLD2. a. Primers 2199/2184 amplify AhLOX7_5' on both progenitor genomes and the tetraploid. Amplicons cover partial E2, partial E4, entire E3, I2, and I3. b. Primers 1991/1992 amplified AhPLD2 in tetraploid and diploid progenitor species; primers 2120/2121 amplified AhPLD1 in both sub-genomes; primers 2187/2188 amplified AhLOX7_3' in both sub-genomes, amplicons contain partial E7, E9, entire E8, I7, and I8; primers 2186/2188 amplified AhLOX7_3' in both sub-genomes at a slightly different amplification start position resulted in similar amplification. DNA size standard: All-purpose Hi-Lo DNA marker, Bionexus, catalogue no. BN2050. TR = A. hypogaea cv. Tifrunner, Ad-A. duranensis (A genome), Ai = A. ipaensis (B genome).

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BMC Genomics

ISSN: 1471-2164

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