Figure 7

Mis-annotation for Refseq on rheMac3. For transcript NM_001260832: A) The 12^th exon has a 2 bp shift between the annotation of RefSeq-rheMac3 and GASS. B) The 12^th intron cannot meet the “GT-AG” canonical splicing site. C) The amino acids coded with triple codon from nucleotides sequence of RefSeq transcript are inconsistent with the amino acid sequences from RefSeq protein database. D) Three RNA-Seq datasets and three DNA-Seq datasets are mapped to the sequences from the 12^th exon and 13^th exon, “AG” cannot be mapped to RNA-Seq datasets and DNA-Seq datasets, meaning that the assembly for the 12^th intron is wrong. E) The corrected genome sequences and annotations are given, and the transcript nucleotide sequences and the codon amino acid sequences are consistent.