Expression levels of candidate IRs in male
antennae measured in single-end
RNA-seqence (A) and RT-qPCR (B). In single-end
RNA-seqence, vertical axis means the log scale of RPKM value to
10. The significant difference between female and male was
justified by method of Audic and Claverie (1997) and indicated
by symbol “*” (FDR < 0.01 and P < 0.05). In RT-qPCR,
vertical axis means log scale of female gene expression to 10.
Female gene expression was calculated by the
2-∆∆Cq algorithm with male as
control and GAPDH and
UCCR as reference genes.
The significant difference was ascertained by Students t test. Symbol “*” means the
significant difference between female and male (P < 0.05).
Error bars signify SD”.