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Table 1 Pichia pastoris cultivations in buffered synthetic media supplemented with different carbon substrates

From: Pichia pastoris regulates its gene-specific response to different carbon sources at the transcriptional, rather than the translational, level

Condition ID Start-OD 600 Cultivation substrate Cultivation time [h] Harvest-OD 600 μ [h −1 ] Bioprocess Step Replicates
Excess glucose D 0.1 2% glucose 23.3 10.0 (1.0) 0.23 (0.004) Glucose batch 3
Excess glycerol G 0.1 2% glycerol 23.3 10.5 (1.3) 0.23 (0.001) Glycerol batch 3
Methanol feed M 1.5 0.5 and 0.6% methanol 24.5 8.6 (1.4) 0.10 (0.008) Methanol shot/feed 3
Limiting glucose X 1.5 0.25% glucose and feed beads 16.8 11.4 (0.6) 0.010 - 0.022 Glucose fed batch 3
  1. Cultures with different biomass densities were fed with appropriate amounts of carbon substrate in order that the cells could be harvested at a similar OD600 [mean (sd)]. Growth rates (μ) [mean (sd)] were recorded; the values were highly reproducible and reflect growth of typical bioprocess phases, as shown.