Pichia pastoris regulates its gene-specific response to different carbon sources at the transcriptional, rather than the translational, level

Table 1 Pichia pastoris cultivations in buffered synthetic media supplemented with different carbon substrates

Condition	ID	Start-OD ₆₀₀	Cultivation substrate	Cultivation time [h]	Harvest-OD ₆₀₀	μ [h ⁻¹ ]	Bioprocess Step	Replicates
Excess glucose	D	0.1	2% glucose	23.3	10.0 (1.0)	0.23 (0.004)	Glucose batch	3
Excess glycerol	G	0.1	2% glycerol	23.3	10.5 (1.3)	0.23 (0.001)	Glycerol batch	3
Methanol feed	M	1.5	0.5 and 0.6% methanol	24.5	8.6 (1.4)	0.10 (0.008)	Methanol shot/feed	3
Limiting glucose	X	1.5	0.25% glucose and feed beads	16.8	11.4 (0.6)	0.010 - 0.022	Glucose fed batch	3

Cultures with different biomass densities were fed with appropriate amounts of carbon substrate in order that the cells could be harvested at a similar OD₆₀₀ [mean (sd)]. Growth rates (μ) [mean (sd)] were recorded; the values were highly reproducible and reflect growth of typical bioprocess phases, as shown.

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