Phylogenetic relationships, gene structures and motif compositions of
family members in
(Pt). Multiple alignment of Hsf (A) and sHsp (B) proteins from A. thaliana (At) and P. trichocarpa (Pt) was performed using MEGA 5.0 by the Neighbor-Joining (NJ) method with 1000 bootstrap replicates (left panel). Exon/intron structures of the Hsf and sHsp genes are shown in the middle panel. Green boxes represent exons and black lines represent introns. The numbers indicate the splicing phases of the Hsf and sHsp genes: 0, phase 0; 1, phase 1; and 2, phase 2. A schematic representation of conserved motifs (obtained using MEME) in Hsf and sHsp proteins is displayed in the right panel. Different motifs are represented by different colored boxes. Details of the individual motifs are in the Additional file 4: Table S4 and Additional file 5: Table S5.