Analysis of the effects of PRMT5 and PRMT4s on constitutive pre-mRNA splicing. (A) Overlap between introns with increased retention in both mutants. (B) Representation Factor of introns whose retention increased simultaneously in prmt5 and prmt4a;4b in the selected functional categories. *: indicates over-representation compared to random, representation factor significance was assessed using a hypergeometric test (p ≤ 0,05). (C) Splicing Ratios of some representative events showing increased inclusion in both mutants is displayed (FDR ≤0,1). The GO category to which each gene belongs is indicated at the bottom of each panel.