Figure 2From: Spiked GBS: a unified, open platform for single marker genotyping and whole-genome profiling Library construction flow chart. GBS libraries are created following standard protocols. Each spiked library amplifies a single target locus. Spiked libraries are pooled, combined with GBS libraries, and sequenced. Sequence data for the amplicon library is parsed using the M13 and unique barcode sequence.Back to article page