Figure 1

Characterization of the postnatal brown to white adipose transformation. (A) Hematoxylin-eosin (HE) staining of perirenal adipose tissue at postnatal days 0, 2, 4, 14 and 30. Representative HE-stained sections are shown for the indicated time points (n = 5). (B) Total RNA was isolated from perirenal adipose tissue and used for RT-qPCR analysis. Relative expression was measured for uncoupling protein 1 (UCP1), type II iodothyronine deiodinase (DIO2) and peroxisome proliferator-activated receptor γ (PPARG) co-activator 1α (PPARGC1A). The mRNA expression levels were normalized to expression of β-actin (ACTB). Data are mean + SEM (n =4-5); *, p < 0.05 vs. day 0. (C) The level of uncoupling protein 1 (UCP1) and ATP synthase subunit β (ATP5B) was determined by immunoblotting on protein pools, one for each day during the time course. Transcription factor IIB (TFIIB) was used as a loading control.