Figure 5From: Global gene expression profiling of brown to white adipose tissue transformation in sheep reveals novel transcriptional components linked to adipose remodeling Expression of selected metabolic enzymes related to fatty acid metabolism. Total RNA was isolated from perirenal adipose tissue and used for RT-qPCR analysis. Relative expression was measured for: (A) carnitine palmitoyltransferase 1b (CPT1B) and hydroxyacyl-CoA dehydrogenase subunit α (HADHA); (B) acetyl-CoA carboxylase (ACACA) and fatty acid synthase (FASN); (C) diacylglycerol O-acyltransferase 1 (DGAT1) and DGAT2. The mRNA expression levels were normalized to expression of β-actin (ACTB). Data are mean + SEM (brown, n = 9; transition, n = 20; white, n = 15); *, p < 0.05.Back to article page