Figure 2

Quantitative metagenomics of serially diluted gut microbiota. A, Scatter plot of gene frequencies derived from quantitative metagenomic profiles of undiluted gut microbiota on the x-axis versus colour coded 10-, 100-, 1000- and 10.000-fold diluted gut microbiota on the y-axis (samples derived from donor #1 gut microbiota). B, Categorical line graph depicts spearman rank correlation coefficients between gene frequencies from metagenomic analysis of undiluted gut microbiota versus gene frequencies of 10-, 100-, 1000- and 10.000-fold diluted gut microbiota from donor #1 (blue) and donor #2 (red). C, Scatter plot of gene frequencies of undiluted samples from the two unrelated donors #1 (x-axis) and #2 (y-axis) are depicted, and their spearman rank correlation is indicated as a dotted line in B, Genes, present in the reference gene catalogue, which are not detected in the samples are excluded from the analysis.