Figure 3

4-mer distribution analysis of raw metagenomic sequences of serially diluted gut microbiota. A, 4-mer abundance distribution (left panel) and individual frequency (right panel) of metagenomic sequences from colour coded dilution series metagenomics of gut microbiota from donor #1 (upper panel) and #2 (lower panel). B, Bar plot visualizes the normalized Shannon Entropy of 4-mer distribution for undiluted and 10-, 100-, 1000- and 10.000-fold diluted gut microbiota metagenomics from donor #1 (blue) and #2 (red). C, Scatter plots depict the correlation between 4-mer distributions of metagenomic sequences from undiluted gut microbiota (y-axis) and 4-mer distributions of metagenomic sequences from 10-, 100-, 1000- and 10.000-fold diluted gut microbiota (x-axis) for donor #1 (upper panel) and #2 (lower panel).