Figure 4

4-mer distribution of microbiota metagenomes correlates with gene mapping efficiency to a reference gene catalogue. A, Line graphs depict the frequency of gene mapping to a reference gene catalogue as a function of the normalized Shannon Entropy of 4-mer distributions for undiluted and 10-, 100-, 1000- and 10.000-fold diluted gut microbiota metagenomics from donor #1 (blue) and #2 (red). B, Scatter plot illustrates the association between normalized Shannon Entropy of 4-mer distributions and the frequency of gene mapping to a reference gene catalogue for 52 gut microbiota metagenomic profiles stratified according to small (red dots, <10¹⁰ bacteria) and large (black dots, >10¹⁰ bacteria) sample size. Spearman rank correlation statistics are indicated.